Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

Detection of Cryptosporidium parvum oocysts in bovine feces by monoclonal antibody capture enzyme-linked immunosorbent assay.

A monoclonal antibody enzyme-linked immunosorbent assay (ELISA) was developed to detect Cryptosporidium parvum oocysts in bovine feces. Fecal oocysts were concentrated by centrifugation through Formalin-ethyl acetate solution and captured with monoclonal antibody 18.280.2 reactive with C. parvum oocysts. Captured oocysts were detected with goat anti-oocyst serum, following the addition of a per...

Prevalence of Cryptosporidium spp. oocysts in dairy calves in Kerman, southeastern Iran

Faecal samples from 291 dairy calves from 10 dairy herds in Kerman were examined to investigate theprevalence of Cryptosporidium infection. Cryptosporidium spp. oocysts were identified by using themodified Ziehl-Neelsen staining technique in 63 (21.65%) calves. Calves were grouped according to theirage as follows:

Contamination of Cryptosporidium spp. Oocysts in Raw Vegetables Produced in Koya City, Iraq

Background: Cryptosporidium spp. is one of the most important parasitic pathogen which causes acute gastroenteritis in human. This study aimed to determine and compare the contamination rates of Cryptosporidium oocysts in some common vegetables that are cultured and consumed in Koya City, Iraq. Methods: Totally, 400 vegetable samples were collected randomly from vegetable farms in Koya city du...

Isolation of Yersinia spp. from bovine feces.

Yersinia spp. were sought in 618 fecal samples from cows. Four strains of Yersinia enterocolitica, serotype O:12,26 (one), O:13,7 (two), and O:18 (one); seven strains of Yersinia kristensenii, serotype O:11,24 (five) and O:12,26 (two); and one strain of Yersinia pseudotuberculosis serotype IIB, were isolated. This is the first time that Y. pseudotuberculosis has been isolated from cows in Japan...

Cryptosporidium spp. in new born lambs in IRAN